Analysis Note

ControlMouse cortical neural stem cells (Catalog No. SCR029).

Application

Research CategoryNeuroscience

Immunohistochemistry:
1:200-1:1,000 dilution from a previous lot was used.

Immunocytochemistry:
1:200-1:1,000 dilution from a previous lot was used.

Western blot:
1:200-1:1,000 using ECL.

Optimal working dilutions must be determined by the end user.

Research Sub CategoryNeuronal & Glial MarkersDevelopmental NeuroscienceRNA Binding Protein (RBP)

Detect Musashi-1 using this Anti-Musashi-1 Antibody validated for use in IC, IH & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. Musashi1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi1 is a selective marker for intestinal stem or early lineage cells. Musashi1 interacts with the Notch pathway during asymmetric cell division by binding the 3′ UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi1 is present.

Immunogen

Synthetic peptide, amino acids 5-21 of Musashi.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 03-114; 04-1041

Physical form

ImmunoAffinity Purified

Purified rabbit polyclonal in buffer containing a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Quality

Routinely evaluated by Western Blot on Human Placenta lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Musashi-1 on 10 µg of Human placenta lysates.

Specificity

Reactivity with other species has not been confirmed.

Recognizes Musashi-1.

Storage and Stability

Stable for 1 year at -80°C from date of receipt.

Target description

39 kDa